CREBBP Antibody - Primary antibody, specific to CREBBP, Rabbit IgG

Cat. No.: Ab097687

GRADE & PURITY Validated, ExactAb™, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Chicken, Cow, Dog, Human, Mouse, Pig, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human CREBBP Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

CREBBP Antibody - Primary antibody, specific to CREBBP, Rabbit IgG

Synonyms

KAT3A | MKHK1 | RSTS1 | RSTS antibody | RTS antibody | Rubinstein Taybi syndrome antibody | CBP | RSTS | CBP antibody | CBP_HUMAN antibody | CREB binding protein antibody | CREB-binding protein antibody | Crebbp antibody | Cyclic AMP responsive enhancer b

Specifications & Purity

ExactAb™, Validated, See COA

Biochemical and Physiological Mechanisms

Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts

Host species

Rabbit

Specificity

CREBBP

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CBP.

Positive Control

WB: HEK293 cell lysate. IHC: Human tonsil tissue. IF/ICC: A431 cells.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: CREBBP Antibody (Ab097687)

CREBBP Antibody (Ab097687) - Western Blot
All lanes: CREBBP Antibody (Ab097687) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 265 kDa
Observed band size: 100, 150, 270 kDa
Exposure time: 223.0 s

IHC: CREBBP Antibody (Ab097687)

CREBBP Antibody (Ab097687) - IHC
Immunohistochemical analysis of CBP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with CREBBP Antibody (Ab097687) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: CREBBP Antibody (Ab097687)

CREBBP Antibody (Ab097687) - IF/ICC
Immunofluorescent analysis of CBP staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CREBBP Antibody (Ab097687) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Application

• Application: Dilution info
• IHC:

  1/100 - 1/200;

• WB:

  1/500-1/1000

• IF/ICC:

  1/100-1/500 ;

Associated Targets(Human)

CREBBP Tchem CREB-binding protein (0 Activities)

Discover Target: CREBBP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CREBBP Tchem CREB-binding protein (1602 Activities)

Discover Target: CREBBP

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action