Acute Mononuclear Cell Leukemia (M5) Detection Tests
Acute Mononuclear Cell Leukemia (M5) Detection Tests
This type of clinical symptoms in addition to the general acute leukemia, its prominent manifestations of skin and mucous membrane damage to the skin appear diffuse maculopapular rash, hard nodules, swelling, pustular or exfoliative dermatitis. Gingival hyperplasia, swelling, bleeding and ulceration, necrosis, etc. are more common. Nasal mucosa is infiltrated and causes nasal congestion, decreased sense of smell, and even pharyngeal edema. Suffocation, etc. Acute mononuclear cell leukemia (acute monoaeate momogtic leakemia AMOL) is also known as acute mononuclear cell leukemia Schiling type, divided into two subtypes, namely, M5a (immature type) and M5b (mature type), the experiment from Mudanjiang Medical College, undergraduate 5-year laboratory guide for laboratory tests.
Operation method
Acute Mononuclear Cell Leukemia (M5) Detection Tests
Principle
This type of clinical symptoms in addition to the general acute leukemia, its prominent manifestations of skin and mucous membrane damage to the skin appear diffuse maculopapular rash, hard nodules, swelling, pustular or exfoliative dermatitis. Gingival hyperplasia, swelling, bleeding and ulceration, necrosis, etc. are more common. Nasal mucosa is infiltrated and causes nasal congestion, decreased sense of smell, and even pharyngeal edema. Suffocation, etc. Acute mononuclear cell leukemia (acute monoaeate momogtic leakemia AMOL) is also known as acute monocytic leukemia Schiling type, divided into two subtypes, namely, M5a (immature) and M5b (mature). Move Hematology: For more product details, please visit Aladdin Scientific website.
l. Hemoglobin and red blood cell counts are moderately to severely reduced.
2. Leukocyte counts are low in most cases and are categorized as predominantly pro- and young monocytes, which may account for 30-45% of the total leukocyte count, M5a is predominantly pro- and young monocytes in about 50-90% of the cases, and M5b is predominantly mononuclear.
3. Both types of platelets are severely reduced
Bone marrow picture:
l. Extremely or markedly active myeloproliferation.
2. M5a is characterized by predominantly promyelocytes ≥80% and fewer young monocytes; M5b is characterized by promyelocytes, young monocytes, and monocytes, but the predominance of young monocytes is ≥20%.
3. 1-2 thin and long Auer's vesicles can be seen in M5 leukemia cells.
Morphological characteristics of leukemia cells:
1. Proto-monocytes and naive monocytes are large in size and variable in morphology.
2. the nucleus is small, often sideways, pencil-shaped, horseshoe-shaped "S" shape, kidney-shaped or irregularly shaped nuclear chromatin loose, arranged like a honeycomb, lighter color.
3. the amount of cytoplasm is relatively more, often appear inside and outside the double layer of pulp, there are obvious pseudopods protruding from the edge of the clear, the thickness and number of particles vary.
Cytochemical staining:
l. POX and SB staining: Proto-monocytes were negative and weakly positive while most of the young monocytes were positive.
2. PAS staining: about half of the pro-monocytes are negative, half are weakly positive with fine granular or pink color, while most of the young monocytes are positive.
3. esterase staining: non-specific esterase staining is positive, which can be inhibited by sodium fluoride, among which alpha-naphthyl butyrate esterase (a-NBE) staining has a greater diagnostic value.