Chronic Granulocytic Leukemia Detection Test
Chronic Granulocytic Leukemia Detection Test
Chronic granulocytic leukemia is a leukemic malignant proliferative disease of the coarse cell system, according to the natural course of the disease, chronic granulocytic leukemia can be divided into chronic phase and acute phase. Chronic phase is slow onset, asymptomatic in the early stage, with low-grade fever, fatigue and night sweating, abscess and emaciation after a few months, and progressive anemia with pallor, weakness, palpitation and shortness of breath in the late stage. In the late stage, there will be petechiae, epistaxis, excessive menstruation and gastrointestinal bleeding, etc. Within 1-4 years, 7O% of cases transform into acute leukemia at this time into the acute stage. This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory guide for testing majors.
Operation method
Chronic Granulocytic Leukemia Detection Test
Principle
Chronic granulocytic leukemia is a leukemic malignant proliferative disease of the coarse cell system, according to the natural course of the disease, chronic granulocytic leukemia can be divided into chronic phase and acute phase. Chronic phase is slow onset, asymptomatic in the early stage, with low-grade fever, fatigue and night sweating, abscess and emaciation after a few months, and progressive anemia with pallor, weakness, palpitation and shortness of breath in the late stage. In the late stage, petechiae, epistaxis, excessive menstruation and gastrointestinal bleeding will appear, etc. In 1-4 years, 70% of cases will be transformed into acute leukemia, and then it will enter into the acute stage. Move Hematology: For more product details, please visit Aladdin Scientific website.
1. Erythrocytes and hemoglobin are normal in the early stages and decrease in the late stages. A few nucleated red blood cells, polychromatic red blood cells and spot-colored red blood cells may be seen in the blood film.
2. Leukocyte counts are significantly elevated, usually around 5.0×109/L in the early stage, mostly l00-300×109/L and up to 1000×109/L. The percentage of categorized granulocyte lineage is higher than normal, and the majority of cells in the stages of neutrophils and below. Progenitor + early granulocytes generally do not exceed 10%, but may not appear in the early stages of the disease, basophils can be as high as 10-20%, is one of the characteristics of slow granulocytes, eosinophils and monocytes can also be increased.
3. Thrombocytosis is seen in 1/3-1/2 of the first diagnosed cases, sometimes up to 1000×109/L while it decreases in the late stage of the disease.
Bone marrow image: bone marrow proliferation is extremely active, the ratio of granulocytes/red can be increased to 10-50:l. Bone marrow classification is dominated by granulocytes, with neutrophilic neutrophilic granulocytes, late granulocytes and rod granulocytes predominating, and progranulocytes + early granulocytes can reach 10-15%, basophils, eosinophils, and granulomatous histiocytes (Ferrala's cells) are increased. Sometimes phagocytes similar to Gaucher's cells and aquaporins can be seen. They are formed by phagocytosis of the cell membrane breakdown products of a large number of granulocytes. There is an increase in megakaryocytes, and the bone marrow biopsy shows that the bone marrow tissue is almost entirely infiltrated by leukemia cells. There is no adipose tissue, and myelofibrosis occurs in about 30-40% of cases at different stages of the disease.