β-Glucuronidase, CAS No.9001-45-0

CAS: 9001-45-0 Cat. No.: G755246 EC Number: 232-606-8

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥250 KU/mL; ~15000 U/mg protein (37℃, pH 6.8, phenolphthalein-β-D-glucuronide); ~50 U/mg protein (37℃, pH 7.5, pNP-β-D-glucuronide)

Accession #

P05804

Bioactivity

≥250 KU/mL; ~15000 U/mg protein (37℃, pH 6.8, phenolphthalein-β-D-glucuronide); ~50 U/mg protein (37℃, pH 7.5, pNP-β-D-glucuronide)

Overview

High purity recombinant β-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and analytical testing applications.

PROPERTIES

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW ~ 82,600) 

- Single major band on isoelectric focusing (pI ~ 5.4) 

2. SPECIFICTY: Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates. 

3. PHYSICOCHEMICAL PROPERTIES: 

pH Optima: 5.0-7.5

Temperature Stability: up to 50°C

Temperature Optima: 37°C

4. STORAGE CONDITIONS: The enzyme is supplied in 20 mM Tris.HCl pH 7.5, 50 mM NaCl, 0.1 mM EDTA plus 0.02% (w/v) sodium azide and should be stored at 4°C. For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.8 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use. 

DO NOT FREEZE / THAW. 

Specifications

Product Name

β-Glucuronidase, CAS No.9001-45-0

Synonyms

β-D-Glucuronide glucuronosohydrolase | beta-glucuronidase | beta-D-glucuronidase | uidA | ECK1612 | gurA | gusA | GUS

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥250 KU/mL; ~15000 U/mg protein (37℃, pH 6.8, phenolphthalein-β-D-glucuronide); ~50 U/mg protein (37℃, pH 7.5, pNP-β-D-glucuronide)

Bioactivity

≥250 KU/mL; ~15000 U/mg protein (37℃, pH 6.8, phenolphthalein-β-D-glucuronide); ~50 U/mg protein (37℃, pH 7.5, pNP-β-D-glucuronide)

Accession #

P05804

Predicted molecular weight

82.6 kDa

CAS

9001-45-0

Enzyme Commission Number

3.2.1.31

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥250 KU/mL; ~15000 U/mg protein (37℃, pH 6.8, phenolphthalein-β-D-glucuronide); ~50 U/mg protein (37℃, pH 7.5, pNP-β-D-glucuronide)

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

1. One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one μg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37°C. 2. *One Unit of β

References

1. Bairen Du, Jingjing Zhang, Yanjie Dong, Junwei Wang, Longwen Lei, Rengfei Shi.  (2020)  Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis.  STEROIDS,      [PMID:32603755] [10.1016/j.steroids.2020.108691]

2. Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li, Yongning Wu.  (2018)  Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection.  TALANTA,      [PMID:30172485] [10.1016/j.talanta.2018.07.073]