10-250 kDa, 10/15/20/25/30/40/50/70/100/130/180/250 kDa 10-250 kDa for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Color pre dyed protein molecular weight standard:
1. This product contains 12 highly purified and pre stained recombinant proteins ranging from 10kDa to 250kDa.
2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting.
3. This product has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents.
4. According to the size of the sample well, this product usually requires 5-10 microliters of sample per loading (5μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.
Usage method:
1. After thawing at room temperature, completely dissolve and gently mix well, do not boil.
2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images.
3. Unused product should be stored under storage conditions and can be left at 4°C for 2 months.
Precautions:
1. In low concentration gels, low molecular weight proteins will swim at the front edge of the dye.
2. When transferring high molecular weight proteins, it is recommended to extend the transfer time or increase the transfer voltage. If the effect is not good, it is recommended to adjust the transfer solution formula, reduce the amount of methanol used, and add a small amount of SDS (final concentration not exceeding 0.04%).
3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.
All Blue Protein MW Marker (10-250kDa) (rp210256) - SDS-PAGE band profile in Tris-Glycine
lmages are from a Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane. All Blue Protein MW Marker (10-250kDa) (rp210256) are mixture of 12 blue-stained recombinant proteins (10-250kDa). These standards can be used for fluorescent blot detection at red wavelengths (635 nm laser or red LED).
All Blue Protein MW Marker (10-250kDa) (rp210256) - SDS-PAGE band profile in Bis-Tris
lmages are from a Bis-Tris gel (SDS-PAGE) and subsequent transfer to membrane. All Blue Protein MW Marker (10-250kDa) (rp210256) are mixture of 12 blue-stained recombinant proteins (10-250kDa). These standards can be used for fluorescent blot detection at red wavelengths (635 nm laser or red LED).
All Blue Protein MW Marker (10-250kDa) (rp210256) - Migration patterns in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein using an All Blue Protein MW Marker (10-250kDa) (rp210256) in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View 10-250 kDa grade guide →
