Cell Permeabilization Buffer (Triton™ X-100) (10×) - BioReagent,sterile-filtered,10×

Cat. No.: F1508364

GRADE & PURITY Sterile-filtered, BioReagent, 10×

Synonyms

Intracellular Staining Permeabilization Wash Buffer (10×) | Permeabilization Buffer | Intracellular Staining Permeabilization Wash Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Flow Cytometry

Overview

Product Introduction

Cell Permeabilization Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Cell Permeabilization Buffer is used to permeabilize cells following fixation. It is supplied as a 10× solution and should be diluted in deionized water prior to use.

Key Components:

• Phosphate Buffered Saline (PBS)

• Triton™ X-100: 3% (v/v)

• Proclin 300: 0.05% (v/v) (as preservative)

Usage Protocol:

Centrifuge fixed cells and remove supernatant. Dilute Cell Permeabilization Buffer (10×) to 1× in DI water.  Add diluted Cell Permeabilization Buffer directly to fixed cells, using approximately 100 µl per 1 million cells. Incubate for 10 min at room temperature. Cells may be washed with phosphate-buffered saline (PBS) to remove Cell Permeabilization Buffer prior to antibody incubation, but it is not necessary.

Precautions:

1、The Triton™ X-100 detergent can reduce cell pellet adhesion, so care should be taken to retain cells when removing supernatant following centrifugation.

2、It is recommended that the reagent be titrated for optimal performance for each application.

Specifications

Synonyms

Intracellular Staining Permeabilization Wash Buffer (10×) | Permeabilization Buffer | Intracellular Staining Permeabilization Wash Buffer

Specifications & Purity

BioReagent, sterile-filtered, 10×

Stability And Storage

Store at 2-8℃ long term (12 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered

Application

• Application: Dilution info
• Flow Cytometry:
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