High Performance Resistance Liquid Chromatography
High Performance Resistance Liquid Chromatography
High performance resistive liquid chromatography, also known as volumetric resistive chromatography (SEC), molecular sieve chromatography, gel filtration, etc. Gel filtration is commonly used in the biochemical industry.SEC is a chromatographic method that separates solute molecules purely according to the size of the solute molecules in the mobile phase solvent, and the packing has a certain range of pore sizes, so that macromolecules can not enter and flow out of the chromatographic column first, and small molecules flow out later. The traditional SEC packing used for the separation of biomolecules is mainly polysaccharide polymer soft gel, which can only be used for slow separation at low pressure. They have been largely replaced by particulate cross-linked hydrophilic gels (e.g., cross-linked agarose Superose 6 and 12), vinyl copolymers (e.g., TSK-Gel PW), and hydrophilic bonded silica gels (e.g., Zorbax GF 250 and 450). The molecular weight classes that can be separated by SEC range from 10,000 to 2,000,000 depending on the pore size of the filler used.
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