L-Glutamic Dehydrogenase (NADP), CAS No.9029-11-2

CAS: 9029-11-2 Cat. No.: R1507821

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥2000U/ml; ≥400U/mg protein

Accession #

P00370

Bioactivity

≥2000U/ml; ≥400U/mg protein

Overview

Glutamate dehydrogenase (NADP) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

Reaction

Product description

Appearance:	Colorless transparent liquid
Source:	Microorganism
Enzyme Commission Number:	EC 1.4.1.4
CAS Number:	9029-11-2
Storage temperature:	2-8℃
Specific activity:	≥400 U/mg protein
Unit definition:	One unit will convert one micromole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30℃.

Properties

Stability:	Stable at 2-8℃ for at least one year
Molecular weight:	51kDa (SDS-PAGE)
Isoelectric point:	4.6
Michaelais constant:	1.7×10⁻³M (NH₄)1.1×10⁻³M (α-Ketoglutarate)3.0×10⁻⁵M (NADPH)
Optimum pH:	8.3	[Fig. 1]
Optimum temperature:	55℃-60℃	[Fig. 3]
pH Stability:	6.0-9.5 (25℃, 20hr)	[Fig. 2]
Thermal stability:	<60℃ (pH7.4, 10min)	[Fig. 4]
Inhibitors:	Ca²⁺, Fe²⁺, Mn²⁺, Zn²⁺, NEM, Proclin, SDS
Effect of various chemicals:		[Table 1]

Specifications

Product Name

L-Glutamic Dehydrogenase (NADP), CAS No.9029-11-2

Synonyms

Glutamate Dehydrogenase (NADP-dependent) | L-Glutamate:NADP+ oxidoreductase (deaminating) | glutamate dehydrogenase (NADP(+)) | glutamic dehydrogenase | gdhA

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥2000U/ml; ≥400U/mg protein

Bioactivity

≥2000U/ml; ≥400U/mg protein

Accession #

P00370

Predicted molecular weight

51kDa (SDS-PAGE)

CAS

9029-11-2

Enzyme Commission Number

EC 1.4.1.4

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥2000U/ml; ≥400U/mg protein

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (12 months). Upon receipt, it is recommended to aliquot.

Unit definition

One unit will convert one micromole of α-keloglutarate to Lglutamate per min at pH 8.3 at 30℃.