Validated, ExactAb™, See COA ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human AKT(phospho S473) Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.
AKT(phospho S473) Antibody (Ab087899) - Western Blot
All lanes: AKT(phospho S473) Antibody (Ab087899) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 16.1 s
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899) (red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899)(red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AKT(phospho S473) Antibody (Ab087899). The picture on the right is blocked with the phospho peptide.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC | 1/50 - 1/200 |
| IHC | 1/100-1/300 |
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Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0707776 | Certificate of Analysis | Apr 17, 2026 | Ab087899 |
| ZJ24F0707777 | Certificate of Analysis | Apr 17, 2026 | Ab087899 |
| ZJ25F0319278 | Certificate of Analysis | Jan 20, 2026 | Ab087899 |
| ZJ25F0319279 | Certificate of Analysis | Jan 20, 2026 | Ab087899 |
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