Binuclease, CAS No.9025-65-4

CAS: 9025-65-4 Cat. No.: B401536 EC Number: 964-102-5
AVAILABLE TO ORDER
GRADE & PURITY Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. Endotoxin tested ? Endotoxin-tested — each lot assayed and labeled with its endotoxin level. Use when you need a documented endotoxin value for risk assessment. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. expressed in Pichia pastoris,Protein Content ≥95% (Biuret test); ≥20 KU/mg enzyme powder
Accession #
P13717
Expression system
Pichia pastoris
Bioactivity
≥20 KU/mg enzyme powder
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
20KU
B401536-20KU
2
$99.90
100KU
B401536-100KU
1
$299.90
500KU
B401536-500KU
1
$999.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Bioactive,Recombinant,ActiBioPure™,endotoxin tested,High Performance,EnzymoPure™,expressed in Pichia pastoris,Protein Content ≥95% (Biuret test); ≥20 KU/mg enzyme powder ActiBioPure™,Bioactive,Endotoxin tested,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Product Name
Binuclease, CAS No.9025-65-4
Synonyms
Serratia marcescens broad-spectrum nuclease | non-restrictive endonuclease | Serratia marcescens nuclease | endonuclease (Serratia marcescens) | Serratia marcescens broad-spectrum nuclease | non-restrictive endonuclease
Grade
ActiBioPure™, Bioactive, Endotoxin tested, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, endotoxin tested, High Performance, EnzymoPure™, expressed in Pichia pastoris,Protein Content ≥95% (Biuret test); ≥20 KU/mg enzyme powder
Biochemical and Physiological Mechanisms
Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA. Miscellaneous: The acti
Bioactivity
≥20 KU/mg enzyme powder
Accession #
Predicted molecular weight
27.9 kDa
CAS
9025-65-4
Enzyme Commission Number
3.1.30.2
Molecule Type
Enzyme
Storage and Shipping
Concentration
expressed in Pichia pastoris,Protein Content ≥95% (Biuret test); ≥20 KU/mg enzyme powder
Reconstitution
Reconstitute in 20 mM Tris-HCl, 20 mM NaCl, 2 mM MgCl₂, pH 8.0.
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
Unit definition
The acid-soluble oligonucleotide fragment produced by reacting with the salmon sperm DNA substrate at pH 8.0 and 37°C for 30 minutes is defined as one unit of nuclease activity with ΔA260nm=1.0 in the spectrophotometric detection.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

39 results found

Lot NumberCertificate TypeDateItem
G2320234Certificate of AnalysisMay 09, 2026 B401536
C2610441Certificate of AnalysisMar 07, 2026 B401536
C2610450Certificate of AnalysisMar 07, 2026 B401536
A2628341Certificate of AnalysisJan 23, 2026 B401536
K2528275Certificate of AnalysisNov 21, 2025 B401536
K2528274Certificate of AnalysisNov 21, 2025 B401536
K2528273Certificate of AnalysisNov 21, 2025 B401536
I2501223Certificate of AnalysisAug 27, 2025 B401536
H2528198Certificate of AnalysisAug 16, 2025 B401536
H2528199Certificate of AnalysisAug 16, 2025 B401536
D2502188Certificate of AnalysisMar 29, 2025 B401536
D2502186Certificate of AnalysisMar 29, 2025 B401536
D2502187Certificate of AnalysisMar 29, 2025 B401536
L2424410Certificate of AnalysisDec 17, 2024 B401536
L2423035Certificate of AnalysisDec 17, 2024 B401536
L2424409Certificate of AnalysisDec 17, 2024 B401536
G2419472Certificate of AnalysisJul 11, 2024 B401536
G2419459Certificate of AnalysisJul 11, 2024 B401536
G2402174Certificate of AnalysisJun 27, 2024 B401536
G2408541Certificate of AnalysisJun 27, 2024 B401536
G2408555Certificate of AnalysisJun 27, 2024 B401536
G2408556Certificate of AnalysisJun 27, 2024 B401536
C2420329Certificate of AnalysisMar 15, 2024 B401536
C2420327Certificate of AnalysisMar 15, 2024 B401536
C2420325Certificate of AnalysisMar 15, 2024 B401536
A2410122Certificate of AnalysisDec 26, 2023 B401536
A2417016Certificate of AnalysisDec 26, 2023 B401536
A2410116Certificate of AnalysisDec 26, 2023 B401536
K2302412Certificate of AnalysisOct 24, 2023 B401536
K2302413Certificate of AnalysisOct 24, 2023 B401536
G2320235Certificate of AnalysisJun 30, 2023 B401536
G2320233Certificate of AnalysisJun 30, 2023 B401536
G2320232Certificate of AnalysisJun 30, 2023 B401536
E2323013Certificate of AnalysisMay 16, 2023 B401536
E2323012Certificate of AnalysisMay 16, 2023 B401536
E2323011Certificate of AnalysisMay 16, 2023 B401536
C2226693Certificate of AnalysisMar 05, 2022 B401536
C2226692Certificate of AnalysisMar 05, 2022 B401536
B2221368Certificate of AnalysisDec 25, 2021 B401536

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Documents & Articles
Citations of This Product
References
1. Yu Li, Miaomiao Cai, Wenwen Zhang, Ying Liu, Xiaoqing Yuan, Na Han, Jing Li, Shengnan Jin, Chunming Ding.  (2023)  Cas12a-based direct visualization of nanoparticle-stabilized fluorescence signal for multiplex detection of DNA methylation biomarkers.  BIOSENSORS & BIOELECTRONICS,      [PMID:37924654] [10.1016/j.bios.2023.115810]
2. Ruopeng Yin, Tao Wang, Jingzu Sun, Huanqin Dai, Yuting Zhang, Ningning Liu, Hongwei Liu.  (2024)  Postbiotics From Lactobacillus Johnsonii Activates Gut Innate Immunity to Mitigate Alcohol-Associated Liver Disease.  Advanced Science,      [PMID:39574408] [10.1002/advs.202405781]
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View Bioactive grade guide → View Recombinant grade guide → View ActiBioPure™ grade guide → View Endotoxin tested grade guide → View High Performance grade guide → View EnzymoPure™ grade guide →

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