Bst DNA Polymerase, Large Fragment (powder)

Cat. No.: B744267
AVAILABLE TO ORDER
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. Store at -20℃ long term (24 months). Avoid freeze/thaw cycle.
Accession #
Q45458
Expression system
E. coli
Bioactivity
One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTP into acid insoluble material in 30 minutes at 65℃
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
4KU
B744267-4KU
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$179.90
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free,Carrier Free,Bioactive,DNase, RNase free,ActiBioPure™,EnzymoPure™,sterile,Store at -20℃ long term (24 months). Avoid freeze/thaw cycle. ActiBioPure™,Animal Free,Bioactive,Carrier Free,DNase, RNase free,Sterile,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Product Name
Bst DNA Polymerase, Large Fragment (powder)
Synonyms
(Bacillus stearothermophilus, Bst) DNA Polymerase I , Large Fragment | DNA polymerase I | polA
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, DNase, RNase free, Sterile, EnzymoPure™
Specifications & Purity
Animal Free, Carrier Free, Bioactive, DNase, RNase free, ActiBioPure™, EnzymoPure™, sterile, Store at -20℃ long term (24 months). Avoid freeze/thaw cycle.
Bioactivity
One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTP into acid insoluble material in 30 minutes at 65℃
Accession #
Molecule Type
Enzyme
Storage and Shipping
Concentration
Store at -20℃ long term (24 months). Avoid freeze/thaw cycle.
Reconstitution
Reconstitute in 10mM Tris-HCl (pH7.5), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50%(v/v) glycerol.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (24 months). Avoid freeze/thaw cycle.
Unit definition
One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTP into acid insoluble material in 30 minutes at 65ºC。

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

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