Coelenterazine 400 a - ≥95% , CAS No.70217-82-2

CAS: 70217-82-2 Cat. No.: C332017 Molecular Weight: 391.46

GRADE & PURITY ≥95%

Synonyms

Coelenterazine 400 a is known as a bisdeoxy derivative of coelenterazine. | BlueSyn C | 2,8-dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3-ol | coelenterazine-400a | 2,8-dibenzyl-6-phenyl-7H-imidazo[1,2-a]pyrazin-3-one | AKOS030213259 | C-7010 | DTXSID10628592

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Overview

product description：

Coelenterazine 400 a is a chromophore that produces the chemiluminescence displayed by the Aequorin protein in the presence of Ca|2+|. Aequorin is employed as a chemiluminescent probe for intracellular Ca|2+|, and derivatives of this protein generated with various Coelenterazine derivatives produced a range of Ca|2+|probes with varying spectral properties. Coelenterazine 400 a is a bisdeoxy derivative of coelenterazine. Coelenterazine 400 a displays an emission maximum of 395nm following conversion by Renilla luciferase (Rluc). Coelenterazine 400 a is commonly paired with class 1 and 3 GFP acceptors.

Applications：

Coelenterazine 400 a is a blue emitting Rluc substrate for bioluminescence resonance energy transfer 2 (BRET2) studies.

Specifications

Synonyms

Coelenterazine 400 a is known as a bisdeoxy derivative of coelenterazine. | BlueSyn C | 2, 8-dibenzyl-6-phenylimidazo[1, 2-a]pyrazin-3-ol | coelenterazine-400a | 2, 8-dibenzyl-6-phenyl-7H-imidazo[1, 2-a]pyrazin-3-one | AKOS030213259 | C-7010 | DTXSID10628592

Specifications & Purity

≥95%

Storage

Protected from light, Store at -20°C, Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Stock Solution1. Let the vial warm to room temperature before opening to prevent condensation from entering the product.2. Dissolve the product in ethanol at a concentration of 0.4 mg/ml. Mix gently.3. Avoid solvents such as DMSO as they may cause oxidati

Purity

≥95%

Names and Identifiers

• Pubchem Sid: 504773439
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504773439
• Canonical Smiles: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• IUPAC Name: 2,8-dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3-ol
• InChIKey: XNNYOKUWNAAJQW-UHFFFAOYSA-N
• INCHI: 1S/C26H21N3O/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21/h1-15,18,30H,16-17H2
• Isomeric SMILES: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• Molecular Weight: 391.46
• Reaxy-Rn: 36019358
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=36019358&ln=

Chemical and Physical Properties

• Solubility: Soluble in 0.1% warm acetone. Hardly soluble in water.
• Refractive Index: n20D1.66 (Predicted)
• Boil Point(°C): 551.39° C at 760 mmHg (Predicted)
• Melt Point(°C): 94-98° C
• Molecular Weight: 391.500 g/mol
• XLogP3: 6.200
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 5
• Exact Mass: 391.168 Da
• Monoisotopic Mass: 391.168 Da
• Topological Polar Surface Area: 50.400 Ų
• Heavy Atom Count: 30
• Formal Charge: 0
• Complexity: 524.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Liu Wang, Yangyi Liu, Jing Sun, Juanjuan Su, Jing Feng, Li Miao, Weijie Zhao, Hongjie Zhang, Kai Liu.  (2025)  In Situ Ultrasound-Triggered Bioluminescence for Combined Sono/Photodynamic Immunotherapy.  ACS Nano,      [PMID:40671351] [10.1021/acsnano.5c06999]