Deoxyribonuclease I, CAS No.9003-98-9

CAS: 9003-98-9 Cat. No.: D128586 EC Number: 232-667-0

GRADE & PURITY Recombinant, EnzymoPure™, ≥5000 units/mg protein;from bovine pancreas

Accession #

P00639

Overview

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

Specifications

Product Name

Deoxyribonuclease I, CAS No.9003-98-9

Grade

Recombinant, EnzymoPure™

Specifications & Purity

Recombinant, EnzymoPure™, ≥5000 units/mg protein;from bovine pancreas

Biochemical and Physiological Mechanisms

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both

Accession #

P00639

CAS

9003-98-9

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥5000 units/mg protein;from bovine pancreas

Storage

Store at 2-8°C

Shipped In

Wet ice