Deoxyribonuclease I, CAS No.9003-98-9

CAS: 9003-98-9 Cat. No.: D128591 EC Number: 232-667-0

GRADE & PURITY EnzymoPure™, from bovine pancreas；≥2,000 units/mg dry weight；无Ribonuclease & Protease

Accession #

P00639

Overview

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.

Specifications

Product Name

Deoxyribonuclease I, CAS No.9003-98-9

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, from bovine pancreas；≥2,000 units/mg dry weight；无Ribonuclease & Protease

Biochemical and Physiological Mechanisms

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both

Accession #

P00639

CAS

9003-98-9

Molecule Type

Enzyme

Storage and Shipping

Concentration

from bovine pancreas；≥2,000 units/mg dry weight；无Ribonuclease & Protease

Storage

Store at 2-8°C

Shipped In

Wet ice