c-Kit/CD117 Rat mAb (AF647) - Primary antibody, specific to c-Kit/CD117

Cat. No.: Ab209966

GRADE & PURITY Validated, ExactAb™, Ex:650nm, Em:668nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

ACK2

Conjugation

AF647

Overview

Rat anti Mouse c-Kit/CD117 Antibody, Monoclonal (ACK2), could be used for Flow, IP, in vivo mast cell depletion, in vivo c-Kit+ cell depletion, in vitro c-Kit neutralization and so on.

Specifications

Product Name

c-Kit/CD117 Rat mAb (AF647) - Primary antibody, specific to c-Kit/CD117

Synonyms

W | Bs | Fdc | Ssm | SCO1 | SCO5 | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody | AF647 anti-mouse CD117 (c-kit) Antibody | CD117 (c-Kit) Monoclonal Antibody (ACK2), AF647 | AF647 Anti-Mouse CD117/c-Kit (ACK2) | v kit Hardy Zucke

Specifications & Purity

ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration a

Host species

Rat

Specificity

c-Kit/CD117

Immunogen

IL-3-dependent mast cells

Positive Control

Flow: BALB/C mouse bone marrow cells

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

Em:668nm, Ex:650nm, ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: c-Kit/CD117 Rat mAb (AF647) (Ab209966)

c-Kit/CD117 Rat mAb (AF647) (Ab209966) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit/CD117 expression on BALB/C mouse bone marrow cells. BALB/C mouse bone marrow cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Rat IgG (AF647) (Ab190071; Left panel) or c-Kit/CD117 Rat mAb (AF647) (Ab209966; Right panel) at 0.2μg/test.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

KIT Tclin Mast/stem cell growth factor receptor Kit (0 Activities)

Discover Target: KIT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)