CD127 Rat mAb (Biotin) - Primary antibody, specific to CD127, Rat IgG2a

Cat. No.: Ab185388

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

A7R34

Conjugation

Biotin

Overview

Application:
Flow: ≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD127 Rat mAb (Biotin) - Primary antibody, specific to CD127, Rat IgG2a

Synonyms

Biotin Anti-Mouse CD127/IL-7RA Antibody[A7R34] | CD127 Monoclonal Antibody (A7R34), Biotin | Biotin Rat Anti-Mouse CD127 | Biotin anti-mouse CD127 (IL-7Rα) Antibody | Interleukin-7 receptor subunit alpha antibody | Interleukin 7 receptor isoform H5 6 anti

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

CD127

Immunogen

IL-7Ra-IgG1 fusion protein.

Positive Control

Flow: Murine splenocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD127 Rat mAb (Biotin) (Ab185388)

CD127 Rat mAb (Biotin) (Ab185388) - Flow Cytometry
Flow cytometry analysis of CD127 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD127 Rat mAb (Biotin) (Ab185388) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD127 Rat mAb (Biotin) (Ab185388)

CD127 Rat mAb (Biotin) (Ab185388) - Flow Cytometry
Flow cytometry analysis of CD127 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD127 Rat mAb (Biotin) (Ab185388) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

IL7R Tbio Interleukin-7 receptor subunit alpha (0 Activities)

Discover Target: IL7R

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)