CD3 Maus-mAb (Biotin) - Primärer Antikörper, specific to CD3, Mouse IgG1

Cat. No.: Ab189658

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

UCHT1

Conjugation

Biotin

Übersicht

Der monoklonale Maus-Anti-Human-CD3-Antikörper (UCHT1) kann für Flow, In-vivo-T-Zelldepletion in humanisierten Mäusen, Ex-vivo-T-Zellhemmung für Xenographien usw. verwendet werden

Specifications

Product Name

CD3 Maus-mAb (Biotin) - Primärer Antikörper, specific to CD3, Mouse IgG1

Synonyme

CD3epsilon | IMD18 | CD3 epsilon | CD3e-Antigen | CD3e-Antigen, Epsilon-Polypeptid (TiT3-Komplex) | CD3e-Molekül, Epsilon (CD3-TCR-Komplex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-Zell-Antigen-Rezeptor-Komplex, Epsilon-Untereinheit von T3 | T-Zelloberfl

Spezifikationen & Reinheit

ExactAb™, validiert, 0,5 mg/mL

Biochemische und physiologische Mechanismen

Der CD3-Komplex vermittelt die Signaltransduktion.

Wirtsspezies

Maus

Spezifität

CD3

Immunogen

Human CD3ε

Positive Kontrolle

Flow: Human peripheral blood lymphocytes

Konjugation

Biotin

Note

ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Bei 2-8°C lagern, vor Licht geschützt, einfrieren verboten

Verschickt in

Nasses Eis

Stabilität und Lagerung

Die Antikörperlösung sollte unverdünnt zwischen 2°C und 8°C gelagert und vor längerer Lichteinwirkung geschützt werden. Nicht einfrieren.

Bilder

Flow Cytometry: CD3 Mouse mAb (Biotin) (Ab189658)

CD3 Mouse mAb (Biotin) (Ab189658) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (Biotin) (Ab189658) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD3 Mouse mAb (Biotin) (Ab189658)

CD3 Mouse mAb (Biotin) (Ab189658) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (Biotin) (Ab189658) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow:

  ≤0.5 μg/test, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume ;

Zugehörige Ziele (menschlich)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.