CD3 Mouse mAb (Biotin) - Primary antibody, specific to CD3, Mouse IgG1

Cat. No.: Ab189658

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

UCHT1

Conjugation

Biotin

Overview

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow, in vivo T cell depletion in humanized mice, ex vivo T cell inhibition for xenographs and so on.

Specifications

Product Name

CD3 Mouse mAb (Biotin) - Primary antibody, specific to CD3, Mouse IgG1

Synonyms

CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

The CD3 complex mediates signal transduction.

Host species

Mouse

Specificity

CD3

Immunogen

Human CD3ε

Positive Control

Flow: Human peripheral blood lymphocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD3 Mouse mAb (Biotin) (Ab189658)

CD3 Mouse mAb (Biotin) (Ab189658) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (Biotin) (Ab189658) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD3 Mouse mAb (Biotin) (Ab189658)

CD3 Mouse mAb (Biotin) (Ab189658) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (Biotin) (Ab189658) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow:

  ≤0.5 μg/test, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action