CD3 Mouse mAb (FITC) - Primärer Antikörper, specific to CD3E, Mouse IgG2a

Cat. No.: Ab179704

GRADE & PURITY Validated, ExactAb™, Azide Free, Ex:498nm, Em:517nm, 5μL/test

Application

Flow Cytometry

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2a

Clone Number

HIT3a

Conjugation

FITC

Übersicht

Application:
Flow: 5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Specifications

Product Name

CD3 Mouse mAb (FITC) - Primärer Antikörper, specific to CD3E, Mouse IgG2a

Synonyme

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Spezifikationen & Reinheit

ExactAb™, Validated, Azide Free, Ex:498nm, Em:517nm, 5μL/test

Wirtsspezies

Maus

Spezifität

CD3E

Immunogen

Human thymocytes

Positive Kontrolle

Flow: Jurkat

Konjugation

FITC

Anregung (nm)

Blue Laser(488nm)

Emission (nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Note

Azide Free, Em:517nm, Ex:498nm, ExactAb™, Validated

Isotyp

Mouse IgG2a

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein A purified

Konzentration

5μL/test

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing

Verschickt in

Wet ice

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD3 Mouse mAb (FITC) (Ab179704)

CD3 Mouse mAb (FITC) (Ab179704) - Flow Cytometry
Flow cytometry analysis of CD3 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (FITC) (Ab179704) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  5μl/test

  For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Zugehörige Ziele (menschlich)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 Mouse mAb (FITC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.