CD45R Rat mAb (AF405) - Primary antibody, specific to CD45R, Rat IgG2a

Cat. No.: Ab191658

GRADE & PURITY Validated, ExactAb™, Ex:401nm, Em:422nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Human, Mouse

Isotype

Rat IgG2a

Clone Number

RA3-6B2

Conjugation

AF405

Overview

Rat anti Mouse/Human CD45R Antibody, Monoclonal (RA3-6B2), could be used for Flow.

Specifications

Product Name

CD45R Rat mAb (AF405) - Primary antibody, specific to CD45R, Rat IgG2a

Synonyms

LCA | CD45 | T200 | GP180 | IMD105 | B220 | CD45 antigen | CD45R | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | T200 glycoprotein | T200 leukocyte common antigen | T200receptor

Specifications & Purity

ExactAb™, Validated, Ex:401nm, Em:422nm, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec

Host species

Rat

Specificity

CD45R

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Positive Control

Flow: C57BL/6 mouse splenocytes.

Conjugation

AF405

Excitation (nm)

Violet Laser(405nm)

Emission(nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grade

Em:422nm, Ex:401nm, ExactAb™, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD45R Rat mAb (AF405) (Ab191658)

CD45R Rat mAb (AF405) (Ab191658) - Flow Cytometry
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD45R Rat mAb (AF405) (Ab191658) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).

Flow Cytometry: CD45R Rat mAb (AF405) (Ab191658)

CD45R Rat mAb (AF405) (Ab191658) - Flow Cytometry
Flow cytometry analysis of CD45R expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD45R Rat mAb (AF405) (Ab191658) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action