CD8 alpha Rat mAb (PE-Cy5) - Primary antibody, specific to CD8 alpha

Cat. No.: Ab190210

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

PE-Cy5

Overview

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Specifications

Product Name

CD8 alpha Rat mAb (PE-Cy5) - Primary antibody, specific to CD8 alpha

Synonyms

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Host species

Rat

Specificity

CD8 alpha

Immunogen

Mouse Spleen Cells or Thymocyte Membranes

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

PE-Cy5

Excitation (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission(nm)

670nm

Ex/Em(nm)

Ex:565nm;Em:670nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD8 alpha Rat mAb (PE-Cy5) (Ab190210)

CD8 alpha Rat mAb (PE-Cy5) (Ab190210) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy5) (Ab190210) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy5) (Ab190072).

Flow Cytometry: CD8 alpha Rat mAb (PE-Cy5) (Ab190210)

CD8 alpha Rat mAb (PE-Cy5) (Ab190210) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD8 alpha Rat mAb (PE-Cy5) (Ab190210) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy5) (Ab190072). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)