CD8 alpha Rat mAb - Primary antibody, specific to CD8 alpha, Rat IgG2b

Cat. No.: Ab095356

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

YTS 169.4

Conjugation

Unconjugated

Overview

Rat anti Mouse CD8 alpha Antibody, Monoclonal (YTS 169.4), could be used for in vivo CD8+ T cell depletion, WB, Flow and so on.
Application:
in vivo CD8+ T cell depletion: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.
Protein Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Specifications

Product Name

CD8 alpha Rat mAb - Primary antibody, specific to CD8 alpha, Rat IgG2b

Synonyms

Ly-2 | Ly-B | Ly-35 | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

CD8 alpha

Immunogen

CBA mouse thymocytes

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Rat IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD8 alpha Rat mAb (Ab095356)

CD8 alpha Rat mAb (Ab095356) - Flow Cytometry
Two-color flow cytometric analysis of CD8 alpha expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD8 alpha Rat mAb (Ab095356; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD8 alpha (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Flow Cytometry: CD8 alpha Rat mAb (Ab095356)

CD8 alpha Rat mAb (Ab095356) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha with CD8 alpha Rat mAb (Ab095356) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150). 

Flow Cytometry: CD8 alpha Rat mAb (Ab095356)

CD8 alpha Rat mAb (Ab095356) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha (red) with CD8 alpha Rat mAb (Ab095356) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo CD8+ T cell depletion:

  Use at an assay dependent concentration.;