CD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2b

Cat. No.: Ab181175

GRADE & PURITY Validated, ExactAb™, Azide Free, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

2.43

Conjugation

Biotin

Overview

Application:

Flow: ≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2b

Synonyms

CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell surface glycoprotein CD8 alph

Specifications & Purity

ExactAb™, Validated, Azide Free, 0.5 mg/mL

Host species

Rat

Specificity

CD8 alpha

Immunogen

Mouse CTL clone L3

Positive Control

Flow: Murine splenocytes

Conjugation

Biotin

Grade

Azide Free, ExactAb™, Validated

Isotype

Rat IgG2b

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD8 alpha Rat mAb (Biotin) (Ab181175)

CD8 alpha Rat mAb (Biotin) (Ab181175) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab181175) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD8 alpha Rat mAb (Biotin) (Ab181175)

CD8 alpha Rat mAb (Biotin) (Ab181175) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab181175) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)