EpCAM/CD326 Rat mAb - Primary antibody, specific to EpCAM/CD326, Rat IgG2a

Cat. No.: Ab101835

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

G8.8

Conjugation

Unconjugated

Overview

Rat anti Mouse EpCAM/CD326 Antibody, Monoclonal (G8.8), could be used for Flow, IHC, ICC/IF, WB and so on.

Application

Flow: Use at an assay dependent concentration.

IHC: Use at an assay dependent concentration.

ICC/IF: Use at an assay dependent concentration.

WB: Use at an assay dependent concentration.
Protein Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Specifications

Product Name

EpCAM/CD326 Rat mAb - Primary antibody, specific to EpCAM/CD326, Rat IgG2a

Synonyms

Ly74 | Egp314 | Ep-CAM | EpCAM1 | Tacsd1 | Tacstd1 | 17-1A | 323/A3 | ACSTD1 | antigen identified by monoclonal AUA1 | CD326 antigen | CD326 | Cell surface glycoprotein Trop-1 | chromosome 4, surface marker (35kD glycoprotein) | DIAR5 | EGP | EGP-2 | EGP3

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

EpCAM/CD326

Immunogen

TE-71 murine thymic epithelial cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: EpCAM/CD326 Rat mAb (Ab101835)

EpCAM/CD326 Rat mAb (Ab101835) - Flow Cytometry
Multicolor flow cytometric analysis of EpCAM/CD326 expression on live mouse thymocytes. BALB/c mouse thymocytes were stained with CD8 alpha Rat mAb (APC) (Ab190214), CD4 Rat mAb (FITC) (Ab181110) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or EpCAM/CD326 Rat mAb (Ab101835; Right panel) at 5.0 μg/mL. A bivariate pseudocolor density plot showing the correlated expression of CD326 (EpCAM) expression (or Isotype control staining) was derived from CD4- gated events with the forward and side light-scatter characteristics of viable thymocytes.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IHC:

  Use at an assay dependent concentration.;

• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

Associated Targets(Human)

EPCAM Tbio Epithelial cell adhesion molecule (0 Activities)

Discover Target: EPCAM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action