Seed germination rate is an important symbol for identifying seed quality, and plays an important role in determining the amount of seed to be sown as well as in seed physiological research, and often needs to be measured. However, the general method of determining germination rate takes a long time, especially for the seeds in the deep dormant state, can not be applied, so it is necessary to establish a set of rapid identification of seed viability according to the physiological characteristics of living seeds to overcome the shortcomings of conventional methods. This experiment introduces three types of methods, namely, embryo stripping method, staining method and fluorescence method, which can be chosen according to the specific situation.
Operation method
Experiments for the rapid determination of seed viability by the peeling method
Principle
This method is suitable for determining the viability of dormant seeds. There are many reasons for deep seed dormancy, some of which are due to pericarp or seed coat binding (e.g., impermeable to water and air), and some of which have germination inhibitors in the pericarp, seed coat, or endosperm, and such obstacles can be overcome by removing the pericarp, seed coat, endosperm, or by cutting away a portion of the cotyledons. When the physical or chemical factors preventing germination are removed, the embryo quickly germinates.
Materials and Instruments
Apples, citrus, corn Move I. Materials and equipment For more product details, please visit Aladdin Scientific website.
Liters of mercury
Petri dish, filter paper, blades, tweezers.
1. Materials: Mature seeds of apples, oranges or milky seeds of maize.
2. Equipment: Petri dish, filter paper, blade, tweezers.
3. Reagent: 0.1 % mercuric chloride.
Experimental steps
1. Seed treatment
300 fresh citrus seeds just peeled from ripe fruits, 100 sun-dried citrus seeds, 200 milk-ripened fresh maize seeds, 100 each of sun-dried milk-ripened maize seeds or stale maize seeds stored for more than three years were taken, sterilized with 0.1% mercuric chloride (mercuric chloride) then washed with water sufficiently, and subjected to the following treatments:
1.1 Fresh citrus seeds, untreated, as a control, the
1.2 Citrus fresh seeds, carefully peeled off the seed coat with forceps, leaving the embryo intact, the
1.3 Citrus fresh seeds, cut the seeds in two horizontally with a razor blade, leaving the half with the radicle, taking care that the excised portion should be not less than half of the full length of the seed.
1.4 Sun-dried citrus seeds, treated as in 1.2.
~undefined citrus seeds after sun-drying that is to lose the ability to germinate.
1.5 Fresh milk-ripened corn kernels, without treatment as a control (fresh milk-ripened corn seed kernels cannot germinate due to the presence of germination inhibitors in the endosperm).
1.6 Fresh milk-ripened maize kernels, with the embryo carefully stripped out with forceps.
1.7 Sun-dried milk-ripened maize seed kernels, untreated.
1.8 Stale kernels of maize stored for more than three years, untreated.
2. Observational measurements
After the above treatment, then the seeds or embryos neatly laid in the petri dish with the appropriate size of filter paper, with distilled water to moisten the filter paper, cover it and place it in a warm box at about 30 ℃ for cultivation, and pay attention to replenish the water at any time (but do not add too much water, otherwise it is easy to be moldy due to lack of oxygen), from the next day onwards, the number of germinated seeds counted every day, entered into the table below, and observed continuously for two weeks.