Simple identification experiments of plant oxidizing enzymes

Summary

Oxidases dehydrogenate from metabolites and allow them to combine directly with oxygen to produce water or hydrogen peroxide. Examples include cytochrome oxidase, ascorbate oxidase, and polyphenol oxidase.

Operation method

Simple identification experiments of plant oxidizing enzymes

Principle

Oxidases dehydrogenate from metabolites and allow them to combine directly with oxygen to produce water or hydrogen peroxide. Examples include cytochrome oxidase, ascorbate oxidase, and polyphenol oxidase.

Materials and Instruments

Potato tubers, pea leaves, onions, sweet potatoes, cucumbers, graduated test tubes.
Catechol alcohol solution
Electric stove

Move

I. Materials and equipment

Materials: potato tubers, pea leaves, onions, sweet potatoes and cucumbers, etc., two 20ml graduated test tubes, electric stove

Reagents: 1.5% catechol alcohol solution: 1.5g of catechol was dissolved in enough 95% alcohol solution and fixed to 100ml.

II. Experimental Steps

1. Take a small amount of peeled fresh potato and chop it.

2. Take 2 test tubes, were added to the appropriate amount of crushed potatoes, one of the first boil for several minutes, and then add 10 drops of freshly prepared 1.5% catechol alcohol solution, observe the phenomenon, such as the solution produces brown, that catechol is oxidized, oxidation degree is different, the performance of different colors.

Repeat the above experiment with pea leaves, onions, sweet potatoes and cucumbers, and if any of these materials do not immediately oxidize the catechol solution, do the following experiment.

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