Protocols

Experiments on the observation of photoperiodic phenomena in plants

Summary

Under natural light conditions, plants were subjected to short days, intermittent light periods, and intermittent darkness to understand the effects of alternating daylight and darkness and their lengths on the flowering and fruiting of short-day crops such as soybeans and rice.

Operation method

Experiments on the observation of photoperiodic phenomena in plants

Principle

Many plants are required to undergo a certain photoperiod in order to flower. It is also known that leaves are organs that feel the effects of photoperiod. Under certain photoperiodic conditions, certain specialized metabolites are formed within the leaf, which are transferred to the growing point, leading to the formation of a flower bud at the growing point.

Materials and Instruments

Soybean seedlings Rice seedlings
Black hoods, dark boxes, dark cabinets, dark rooms, fluorescent lights, red bulbs, alarm clocks.

Move

I. Materials and equipment

1. Materials: soybean seedlings (late-maturing species) or rice seedlings (light-sensitive varieties), chrysanthemums or other short-sunlight plants

2. Equipment: black cover (white outside, black inside) or dark box, dark cabinet, dark room, fluorescent lamps or red bulbs (60 to 100 watts), alarm clock (with automatic control device for light source switch).

II. Experimental steps

1. When soybean seedlings grow the first compound leaves, or rice seedlings grow 5 to 6 leaves (night temperature above 20 ℃), that is, according to the following method to give different treatments, generally 10 days after continuous treatment can be completed.

After the above treatments, the bud stage of soybean or the beginning of rice spike stage of each treatment was recorded and compared with the control.

2. Take 4 plants of chrysanthemums or other suitable short-day plants, and treat the first plant with 18 hours of light per day for long days, the second plant with 10 hours of light per day for short days, the third plant with short days for the lower leaves and long days for the tops of the removed leaves, and the fourth plant with the opposite of the third plant. Final flowering was observed and results were recorded.


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Categories: Protocols
Explore topics: Botanical experiments

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Cite this article

Aladdin Scientific. "Experiments on the observation of photoperiodic phenomena in plants" Aladdin Knowledge Base, updated Dec 24, 2024. https://www.aladdinsci.com/us_en/faqs/experiments-on-the-observation-of-photop-en.html
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