Serialization experiments
Serialization experiments
It is a complementary experiment to experimental protocols A and B of the differential display technique (DD) experiment and the gene expression series analysis experiment.
Modern Neuroscience Research Techniques
Author(s): U. Windhorst & H. Johansson Translated by Z. Q. Zhao Jun Chen
Operation method
Serialization experiments
Materials and Instruments
Solution and Buffer LoTE cDNA Label Ligase Move Experimental Programs A and B 1. Cut and purified double tags were joined in tandem: the 2. Incubate for 2 h at 16t℃. 3. Sample the double-labeled tandem onto one lane of an 8% poly(acrylamide) gel and electrophorese for 3 h at room temperature, 80°C, using a 100bpladder as a molecular weight standard. 4. EB staining. 5. Cut off the gel region containing 400bp or larger tandem. 6. Purify the DNA from the excised gel fragments to isolate the double-tagged fragments as described in protocol 8. However, do not incubate at 37°C but at 65°C and do not use a dry ice/ethanol ice bath before centrifugation. 7. After precipitation, rinse the precipitate twice with 70% ethanol, dry and redissolve in 40ul of LoTE. For more product details, please visit Aladdin Scientific website.
