multiplex PCR
Multiplex polymerase chain reaction (MPCR), also known as compound PCR, is a new PCR amplification technique improved and developed on the basis of conventional
Topic: PCR technology
Articles by Topic "PCR technology"
Knowledge Base
Precision amplification experiments for large fragments
Amplify a ~5kb fragment with 10 different primer pairs, the template DNA is the same, the reaction system is 50ul, and the control has no template and contains
Purification of PCR fragments from agarose gels
The Wizard SV Gel and PCR Purification System is a method for the direct purification of PCR fragments from agarose gels or from PCR amplification reaction mixt
Primer extension experiment
Primer extension analysis was used to determine the location and quantify the amount of the 5'-end of a specific RNA. To end the labeled oligonucleotide hybridi
PCR amplification of high GC content fragments
The following program describes in detail the PCR amplification of high GC content antique fragments. This experiment was derived from PCR Laboratory Guide (Sec
Sequencing reaction experiments
To get the right signal intensity, adjust the amount of DNA according to the size of the DNA fragments. For ABI PRISM Big Dye Terminator Ready Reaction mix (App
PCR-SSCP technology experiment
Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) technology can be used for: (1) screening and detection of oncogenes and oncogenic
Experimental purification of PCR products with dUTP
The incorporation of dUTP into the replicon during PCR amplification is one of the most efficient and widely used methods for purifying PCR products (Longoetal.
Restriction fragment length polymorphism (RFLP) analysis
Restriction fragment length polymorphism (RFLP) is one of the most important analytical methods in molecular biology and is mainly used to (1) detect DNA sequen