GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
ExactAb™, Validated, Recombinant, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human MMP9 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
MMP9 antibody - Primary antibody, specific to MMP9, Rabbit IgG
Synonyms
92 kDa gelatinase | 92 kDa type IV collagenase | EC 3.4.24.35 | Gelatinase B | GELB | MMP-9 | 82 kDa matrix metalloproteinase-9 antibody | 92 kDa gelatinase antibody | 92 kDa type IV collagenase antibody | CLG 4B antibody | CLG4B antibody | Collagenase Ty
Specifications & Purity
ExactAb™, Validated, Recombinant, See COA
Biochemical and Physiological Mechanisms
Protein Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C
Host species
Rabbit
Specificity
MMP9
Immunogen
A synthetic peptide derived from human MMP9 (AA 100-165)
Positive Control
WB: U-2 OS treated with PMA , U937 treated with PMA and Brefeldin A cell lysates. IHC: Human colon cancer tissue. IF/ICC: U87-MG cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle
Images
MMP9 antibody (Ab115721) - Western Blot All lanes: MMP9 antibody (Ab115721) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 78 kDa Observed band size: 92 kDa. Exposure time: 5.2 s
MMP9 antibody (Ab115721) - Western Blot All lanes: MMP9 antibody (Ab115721) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 78 kDa Observed band size: 92 kDa. Exposure time: 17.1 s
MMP9 antibody (Ab115721) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MMP9 antibody (Ab115721). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
MMP9 antibody (Ab115721) - IF/ICC Immunofluorescence analysis of MMP9 in U87-MG using MMP9 antibody (Ab115721).
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/50 - 1/100
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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