GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Rabbit anti Human NDUFA9 Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application:
WB: 1/500-1/1000 IHC: 1/50-1/100
IF/ICC: 1/200 Protein Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Specifications
Product Name
NDUFA9 Antibody - Primary antibody, specific to NDUFA9, Rabbit IgG
WB: A431, MCF7, HEK293, K562, Jurkat, Hep G2 and HeLa cell lysates. IHC: Human liver cancer tissue. IF./ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
NDUFA9 Antibody (Ab117346) - Western Blot All lanes: NDUFA9 Antibody (Ab117346) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 43 kDa Observed band size: 34 kDa Exposure time: 1.0 s
NDUFA9 Antibody (Ab117346) - IHC Immunohistochemistry analysis of paraffin-embedded Human liver cancer using NDUFA9 Antibody (Ab117346). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
NDUFA9 Antibody (Ab117346) - IF/ICC IF analysis of NDUFA9 Antibody (Ab117346) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with NDUFA9 Antibody (Ab117346) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
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Reconstitution Calculator
Reviews
Customer Reviews
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