Recombinant GST tag Antibody (Ab106638) - IP
All lanes: Recombinant GST tag Antibody (Ab106638) at 1/2000 dilution
Lane 1: GST-tag Purification Resin (G751554) IP in HEK-293 stably transfected with a P2A-bicistronic vector co-expressing Puromycin Resistance gene and an 11-tag fusion protein (Flag, HA, Myc, S-Tag, GST, His, V5, VSV-G, Strep Tag II, SUMO and EGFP) whole cell lysate.
Lane 2: GST-tag Purification Resin (G751554) IP in HEK-293 whole cell lysate.
Lane 3: HEK-293 stably transfected with a P2A-bicistronic vector co-expressing Puromycin Resistance gene and an 11-tag fusion protein (Flag, HA, Myc, S-Tag, GST, His, V5, VSV-G, Strep Tag II, SUMO and EGFP) whole cell lysate.
Lane 4: HEK-293 whole cell lysate 20μg.
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 115 kDa
Observed band size: 56, 64, 118 kDa
Exposure time: 1.1 s