GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human RIT1/RIT Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/2000 IHC: 1/50-1/300 Protein Function Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.
Specifications
Product Name
RIT1/RIT Antibody - Primary antibody, specific to RIT1, Rabbit IgG
Synonyms
GTP binding protein Roc1 antibody | GTP-binding protein Rit1 antibody | NS8 antibody | Ras like protein expressed in many tissues antibody | Ras like without CAAX 1 antibody | Ras-like protein expressed in many tissues antibody | Ras-like without CAAX pro
Specifications & Purity
ExactAb™, Validated, 1.0 mg/mL
Host species
Rabbit
Specificity
RIT1
Immunogen
A synthetic peptide derived from human RIT1 (AA 170-219).
Positive Control
WB: A431, NIH/3T3 and C6 cell lysates. IHC: Human squamous tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
RIT1/RIT Antibody (Ab125431) - Western Blot All lanes: RIT1/RIT Antibody (Ab125431) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 25 kDa Observed band size: 28 kDa Exposure time: 19.4 s
RIT1/RIT Antibody (Ab125431) - IHC Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. RIT1/RIT Antibody (Ab125431) was diluted at 1/200 (4℃， overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
RIT1/RIT Antibody (Ab125431) - IF/ICC IF analysis of RIT1/RIT Antibody (Ab125431) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with RIT1/RIT Antibody (Ab125431) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1/200
IHC
1/50-1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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