GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Ex:565nm?Fluorescence excitation maximum at 565 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:575nm?Fluorescence emission maximum at 575 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.0.5 mg/mL
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface wi
Host species
Rat
Specificity
Transferrin Receptor/CD71
Immunogen
Mouse erythroleukemia cell line 745.6
Positive Control
Flow: C57BL/6 mouse bone marrow cells
Conjugation
PE
Excitation (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
Grade
Em:575nm, Ex:565nm, ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Transferrin Receptor/CD71 Rat mAb (PE) (Ab190203) - Flow Cytometry Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were stained with Transferrin Receptor/CD71 Rat mAb (PE) (Ab190203) at 0.05μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).
Transferrin Receptor/CD71 Rat mAb (PE) (Ab190203) - Flow Cytometry Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Rat mAb (PE) (Ab190203) at 0.05μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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