Vedolizumab (anti-α4β7-integrin) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to Integrin a4b7 (ITGA4 & ITGB7), >95%, Human IgG1, Antibody of integrin α4β7, Antibody of integrin α4β7

CAS: 943609-66-3 Cat. No.: Ab169270 PubChem CID: 168010198

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Human

Isotype

Human IgG1

Conjugation

Unconjugated

Overview

Vedolizumab is a humanized IgG1 monoclonal antibody that targets the α4β7 integrin for the treatment of ulcerative colitis and Crohn's disease.
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Specifications

Product Name

Vedolizumab (anti-α4β7-integrin) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to Integrin a4b7 (ITGA4 & ITGB7), >95%, Human IgG1, Antibody of integrin α4β7

Synonyms

Recombinant Vedolizumab Antibody | Vedolizumab | Entyvio | Anti-Integrin a4b7 (ITGA4 & ITGB7) Reference Antibody (vedolizumab) | Antigen CD49D antibody | CD49d antibody | IA4 antibody | Integrin alpha 4 subunit antibody | Integrin alpha IV antibody | Inte

Specifications & Purity

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Host species

Human

Specificity

Integrin a4b7 (ITGA4 & ITGB7)

Application

ELISA, Functional Assay, Flow cytometry, Kinetics (BLI), Kinetics (SPR)

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Action Type

INHIBITOR

Mechanism of action

Antibody of integrin α4β7

Antibody Type

Primary antibody

Clonality

Recombinant

Isotype

Human IgG1

Light Chain Type

kappa

SDS-PAGE

26.5 kDa (Light Chain) & 50.8 kDa (Heavy Chain), under reducing conditions; 178.5 kDa, under non-reducing conditions.

Purification Method

Protein A purified

Purity

>95%

Source

CHO supernatant

Concentration

See COA

CAS

943609-66-3

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: Vedolizumab (anti-α4β7-integrin) (Ab169270)

Vedolizumab (anti-α4β7-integrin) (Ab169270) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling α4β7-integrin (red) with Vedolizumab (anti-α4β7-integrin) (Ab169270). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG ( Ab170213 ). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Vedolizumab (anti-α4β7-integrin) (Ab169270)

Vedolizumab (anti-α4β7-integrin) (Ab169270) - SEC
The purity of Vedolizumab (anti-α4β7-integrin) (Ab169270) is more than 95% verified by HPLC.

Associated Targets(Human)

ITGA4 Tclin Integrin alpha-4 (0 Activities)

Discover Target: ITGA4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ITGB7 Tclin Integrin beta-7 (0 Activities)

Discover Target: ITGB7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ITGA4 Tclin Integrin alpha-4 (295 Activities)

Discover Target: ITGA4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

ITGB7 Tclin Integrin beta-7 (3 Activities)

Discover Target: ITGB7

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action