c-Kit/CD117 Rat mAb (Biotin) - Primary antibody, specific to c-Kit/CD117

Cat. No.: Ab209972

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

ACK2

Conjugation

Biotin

Overview

Rat anti Mouse c-Kit/CD117 Antibody, Monoclonal (ACK2), could be used for Flow, IP, in vivo mast cell depletion, in vivo c-Kit+ cell depletion, in vitro c-Kit neutralization and so on.

Specifications

Product Name

c-Kit/CD117 Rat mAb (Biotin) - Primary antibody, specific to c-Kit/CD117

Synonyms

W | Bs | Fdc | Ssm | SCO1 | SCO5 | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody | Biotin anti-mouse CD117 (c-kit) Antibody | CD117 (c-Kit) Monoclonal Antibody (ACK2), Biotin | Biotin Anti-Mouse CD117/c-Kit (ACK2) | v kit Hardy Zu

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration a

Host species

Rat

Specificity

c-Kit/CD117

Immunogen

IL-3-dependent mast cells

Positive Control

Flow: BALB/C mouse bone marrow cells

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: c-Kit/CD117 Rat mAb (Biotin) (Ab209972)

c-Kit/CD117 Rat mAb (Biotin) (Ab209972) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit/CD117 expression on BALB/C mouse bone marrow cells. BALB/C mouse bone marrow cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (Biotin) (Ab180310; Left panel) or c-Kit/CD117 Rat mAb (Biotin) (Ab209972; Right panel) at 0.2μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

KIT Tclin Mast/stem cell growth factor receptor Kit (0 Activities)

Discover Target: KIT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)