CD19 Rat mAb(Biotin) - Primary antibody, specific to CD19, Rat IgG2a

Cat. No.: Ab185392

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

1D3

Conjugation

Biotin

Overview

Flow: ≤0.3 μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD19 Rat mAb(Biotin) - Primary antibody, specific to CD19, Rat IgG2a

Synonyms

Biotin Anti-Mouse CD19 Antibody[1D3] | Biotin Anti-CD19 antibody [1D3] | Biotin Rat Anti-Mouse CD19 | Biotin anti-mouse CD19 Antibody | T-cell surface antigen Leu-12 antibody | MGC12802 antibody | MGC109570 antibody | Leu12 antibody | Leu-12 antibody | Le

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

CD19

Immunogen

Transfected k562 cells expressing the extracellular domain of murine CD19.

Positive Control

Flow: Mouse splenocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD19 Rat mAb (Biotin) (Ab185392)

CD19 Rat mAb (Biotin) (Ab185392) - Flow Cytometry
Flow cytometry analysis of CD19 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD19 Rat mAb (Biotin) (Ab185392) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD19 Rat mAb (Biotin) (Ab185392)

CD19 Rat mAb (Biotin) (Ab185392) - Flow Cytometry
Flow cytometry analysis of CD19 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD19 Rat mAb (Biotin) (Ab185392) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)

Discover Target: CD19

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action