EpCAM/CD326 Rat mAb (AF700) - Primary antibody, specific to EpCAM/CD326

Cat. No.: Ab191763

GRADE & PURITY Validated, ExactAb™, Ex:696nm, Em:719nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

G8.8

Conjugation

AF700

Overview

Rat anti Mouse EpCAM/CD326 Antibody, Monoclonal (G8.8), could be used for Flow, IHC, ICC/IF, WB and so on.

Specifications

Product Name

EpCAM/CD326 Rat mAb (AF700) - Primary antibody, specific to EpCAM/CD326

Synonyms

Ly74 | Egp314 | Ep-CAM | EpCAM1 | Tacsd1 | Tacstd1 | 17-1A | 323/A3 | ACSTD1 | antigen identified by monoclonal AUA1 | CD326 antigen | CD326 | Cell surface glycoprotein Trop-1 | chromosome 4, surface marker (35kD glycoprotein) | DIAR5 | EGP | EGP-2 | EGP4

Specifications & Purity

ExactAb™, Validated, Ex:696nm, Em:719nm, 0.5 mg/mL

Biochemical and Physiological Mechanisms

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Pla

Host species

Rat

Specificity

EpCAM/CD326

Immunogen

TE-71 murine thymic epithelial cells

Positive Control

Flow: C57BL/6 Mouse thymic cells.

Conjugation

AF700

Excitation (nm)

Red Laser(633nm)

Emission(nm)

719nm

Ex/Em(nm)

Ex:696nm;Em:719nm

Grade

Em:719nm, Ex:696nm, ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: EpCAM/CD326 Rat mAb (AF700) (Ab191763)

EpCAM/CD326 Rat mAb (AF700) (Ab191763) - Flow Cytometry
Multicolor flow cytometric analysis of EpCAM/CD326 expression on live mouse thymocytes. C57BL/6 mouse thymocytes were stained with CD8 alpha Rat mAb (FITC) (Ab190208), CD4 Rat mAb (PE) (Ab181111) and either Rat IgG (AF700) (Ab190242; Left panel) or EpCAM/CD326 Rat mAb (AF700) (Ab191763; Right panel) at 0.3 μg/test. A bivariate pseudocolor density plot showing the correlated expression of CD326 (EpCAM) expression (or Isotype control staining) was derived from CD4- gated events with the forward and side light-scatter characteristics of viable thymocytes.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

EPCAM Tbio Epithelial cell adhesion molecule (0 Activities)

Discover Target: EPCAM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)