GRADE & PURITY Validated, ExactAb™, Ex:401nm, Em:422nm, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

W6/32

Conjugation

AF405

Overview

Mouse anti Human HLA Class I Antibody, Monoclonal (W6/32), could be used for WB, Flow, IP, IHC, ICC/IF, Functional Assays and so on.

Specifications

Product Name

HLA Class I Mouse mAb (AF405) - Primary antibody, specific to HLA Class I

Specifications & Purity

ExactAb™, Validated, Ex:401nm, Em:422nm, 5 μL/test

Host species

Mouse

Specificity

HLA Class I

Immunogen

Human tonsil cell membrane

Positive Control

Flow: Human peripheral blood lymphocytes

Conjugation

AF405

Excitation (nm)

Violet Laser(405nm)

Emission(nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grade

Em:422nm, Ex:401nm, ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

5 μL/test

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: HLA Class I Mouse mAb (AF405) (Ab191625)

HLA Class I Mouse mAb (AF405) (Ab191625) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with HLA Class I Mouse mAb (AF405) (Ab191625) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).

Flow Cytometry: HLA Class I Mouse mAb (AF405) (Ab191625)

HLA Class I Mouse mAb (AF405) (Ab191625) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with HLA Class I Mouse mAb (AF405) (Ab191625) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  5 μL/test, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood ;

Associated Targets(Human)

HLA-A Tclin HLA class I histocompatibility antigen, A-3 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action