GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Host species
Rabbit
Specificity
Involucrin
Immunogen
A synthetic peptide derived from human Involucrin (AA 536-585).
Positive Control
WB: A431 cell lysate. IHC: Human breast carcinoma tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Involucrin Antibody (Ab111039) - Western Blot All lanes: Involucrin Antibody (Ab111039) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 68 kDa Observed band size: 110, 29 kDa The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90 - 140 kDa. (PMID: 11099111, 1503502, 12150517).Research also has shown that the C-terminal fragment of involucrin (~30 kDa) can participate in repair through cross-linking in a model of epidermal barrier damage (PMID: 9208334). Exposure time: 120.0 s
Involucrin Antibody (Ab111039) - IHC Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Involucrin Antibody (Ab111039). The picture on the right is blocked with the synthesized peptide.
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/100-1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
