GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.200μg, Mycoplasma free
Carrier Free, Azide Free, 200μg, Mycoplasma free Azide Free,Carrier Free for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product Description：
The NIH/3T3 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
NIH/3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies.
Specifications
Product Name
NIH/3T3 Whole Cell Lysate
Grade
Azide Free, Carrier Free
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Molecule Type
Cell or Tissue Extract
Storage and Shipping
Shape
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
Storage
Store at -80°C
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Images
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 58 kDa
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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