GRADE & PURITYPBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
PBS Only, See COA PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
RRabbit anti Human CEP55 Antibody, Recombinant, could be used for WB, IF/ICC and so on.
Specifications
Product Name
Recombinant CEP55 Antibody - Primary antibody, specific to CEP55
Specifications & Purity
PBS Only, See COA
Biochemical and Physiological Mechanisms
Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
Host species
Rabbit
Specificity
CEP55
Immunogen
A synthetic peptide derived from human CEP55 (AA 17-30)
Positive Control
WB: HeLa cell lysate. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
PBS Only
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CEP55 Antibody (Ab336940) - Western Blot
All lanes: Recombinant CEP55 Antibody (Ab336940) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
Exposure time: 120.0 s
Recombinant CEP55 Antibody (Ab336940) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant CEP55 Antibody (Ab336940) (green), and DAPI (blue).
Recombinant CEP55 Antibody (Ab336940) - IF/ICC
Immunofluorescent analysis of HeLa cells labelling CEP55 with Recombinant CEP55 Antibody (Ab336940).
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Recombinant CEP55 Antibody (Ab336940) (1/200) at room temperature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205), (shown in green). Nuclear DNA was labeled in blue with DAPI (D609734).
Control: Secondary antibody only.
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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