GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥97%(SDS-PAGE)
Expression System
HEK293
Accession #
P80188
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3 .
Animal Free,Carrier Free,Bioactive,ActiBioPure™,Azide Free,His-Tag,≥97%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Purity >97% SDS-PAGE.
Actual molecular weight 25.7 kDa
Molecular weight informat ~ 25.7 kDa (monomer). Human lipocalin-2 (aa 1-198) is fused at the C-terminus to a polyhistidine tag. Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Specifications
Product Name
Recombinant Human Lipocalin-2/NGAL Protein, ＞97%(SDS-PAGE)
Synonyms
24p3 | 25 kDa alpha 2 microglobulin related subunit of MMP9 | 25 kDa alpha-2-microglobulin-related subunit of MMP-9 | Alpha 2 microglobulin related protein | HGNC:6526 | HNL | Lcn 2 | Lcn2 | Lipocalin-2 | Migration stimulating factor inhibitor | MSFI | Ne
Grade
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, His Tag
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3 .
It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Samples are stable for twelve months from date of receipt at -20℃ to -80℃.Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Images
Recombinant Human Lipocalin-2/NGAL Protein (rp148318) - Protein Bioactivity Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2/NGAL Protein (rp148318). Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3.
Iron III (Fe3+): Aladdin, Cat# I115411 2,3,Dihydroxybenzoic Acid (DHBA): Aladdin, Catalog # D154867
Recombinant Human Lipocalin-2/NGAL Protein (rp148318) - SDS-PAGE 3 μg/lane of Recombinant Human Lipocalin-2/NGAL was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 24.6 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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