Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human JNK1/JNK2/JNK3 Antibody, Recombinant, could be used for WB, ICC, IF, IP and so on.
Application:
WB: 1/1000-1/2000
ICC/IF: 1/50-1/200
IP: 1/50
Protein Function：
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - Western Blot
All lanes: Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 46, 54 kDa
Observed band size: 39, 46 kDa
Exposure time: 100.0 s
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - IP
Immunoprecipitate (IP) analysis using Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/50 dilution. (WB with Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/3000 dilution).
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/50 dilution.
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513).
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
|
| WB | 1/1000 - 1/2000 |
| IP |
1/50
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ26F0435046 | Certificate of Analysis | May 08, 2026 | Ab111513 |
| ZJ24F1012514 | Certificate of Analysis | Oct 21, 2024 | Ab111513 |
| ZJ24F1012515 | Certificate of Analysis | Oct 21, 2024 | Ab111513 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →
