GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human SIRT1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC, IP and so on.
Specifications
Product Name
Recombinant SIRT1 Antibody - Primary antibody, specific to SIRT1, Rabbit IgG
Synonyms
2.3.1.- | EC 2.3.1.286 | hSIR2 | hSIRT1 | NAD-dependent protein deacylase sirtuin-1 | Regulatory protein SIR2 homolog 1 | SIR2-like protein 1 | EC 3.5.1 | S. cerevisiae, homolog) 1 | SIR2alpha | SIR2L1 | SIR2-like protein 1 | SIRT1 | Sirtuin 1 | SIR2 | 75
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Host species
Rabbit
Specificity
SIRT1
Immunogen
A synthetic peptide derived from human SIRT1(AA 700-740)
Positive Control
WB: A549, HeLa, K562, MCF7, Jurkat and Hep G2 cell lysates. IHC: Rat stomach, Human pituitary tumor, Human colon, Human placenta, Mouse lung, Human kidney tissue. IF/ICC: HeLa cells. IP: HeLa lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant SIRT1 Antibody (Ab127656) - Western Blot All lanes: Recombinant SIRT1 Antibody (Ab127656) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 82 kDa Observed band size: 116 kDa Exposure time: 155.2 s
Recombinant SIRT1 Antibody (Ab127656) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant SIRT1 Antibody (Ab127656) at 1/50 dilution.
Recombinant SIRT1 Antibody (Ab127656) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant SIRT1 Antibody (Ab127656).
Recombinant SIRT1 Antibody (Ab127656) - IP Immunoprecipitate (IP) analysis using Recombinant SIRT1 Antibody (Ab127656) at 1/50 dilution. (WB with Recombinant SIRT1 Antibody (Ab127656) at 1/6000 dilution)
Recombinant SIRT1 Antibody (Ab127656) - IHC Immunohistochemical analysis of paraffin-embedded Rat stomach, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilu
Recombinant SIRT1 Antibody (Ab127656) - IHC Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.
Recombinant SIRT1 Antibody (Ab127656) - IHC Immunohistochemical analysis of paraffin-embedded Human colon, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.
Recombinant SIRT1 Antibody (Ab127656) - IHC Immunohistochemical analysis of paraffin-embedded Human placenta, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.
Recombinant SIRT1 Antibody (Ab127656) - IHC Immunohistochemical analysis of paraffin-embedded Mouse lung, using Recombinant SIRT1 Antibody (Ab127656) at 1/100 dilution.
Recombinant SIRT1 Antibody (Ab127656) - IHC Immunohistochemical analysis of paraffin-embedded human kidney, using Recombinant SIRT1 Antibody (Ab127656).
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1/50 - 1/200
IHC
1/50-1/200
IP
1/30
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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