GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.See COA
Recombinant, ExactAb™, KD Validation, Validated, See COA ExactAb™,KD Validation,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human STAT3 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant STAT3 Antibody - Primary antibody, specific to STAT3, Rabbit IgG
Synonyms
APRFMGC16063 | DNA-binding protein APRF | FLJ20882 | HIES | signal transducer and activator of transcription 3 (acute-phase responsefactor) | signal transducer and activator of transcription 3 | STAT3 | 1110034C02Rik antibody | Acute-phase response factor
Specifications & Purity
Recombinant, ExactAb™, KD Validation, Validated, See COA
Biochemical and Physiological Mechanisms
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:173442
Host species
Rabbit
Specificity
STAT3
Immunogen
A synthetic peptide derived from human STAT3 (AA 670-700)
Positive Control
WB: Mouse heart, HT-1080, HeLa, HEK293, A375, A431, NIH/3T3 and C6 lysates. IHC: Rat brain, Human astrocytoma, Human Hodgkin's lymphoma tissues. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, KD Validation, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant STAT3 Antibody (Ab129421) - Western Blot All lanes: Recombinant STAT3 Antibody (Ab129421) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 88 kDa Observed band size: 91 kDa Exposure time: 5.2 s
Recombinant STAT3 Antibody (Ab129421) - Western Blot All lanes use Recombinant STAT3 Antibody (Ab129421) at 1/1000 dilution for 1 hour at room temperature.
Recombinant STAT3 Antibody (Ab129421) - IF/ICC Immunofluorescent analysis of Hela cells treated with IFN alpha, using Recombinant STAT3 Antibody (Ab129421).
Recombinant STAT3 Antibody (Ab129421) - IHC Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using Recombinant STAT3 Antibody (Ab129421) at 1/100 dilution.
Recombinant STAT3 Antibody (Ab129421) - IHC Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using Recombinant STAT3 Antibody (Ab129421) at 1/100 dilution.
Recombinant STAT3 Antibody (Ab129421) - IHC Immunohistochemical analysis of paraffin-embedded rat brain, using Recombinant STAT3 Antibody (Ab129421).
Application
Application
Dilution info
IHC
1/100 - 1/200
IF/ICC
1/50 - 1/200
WB
1/1000-1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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