SIRP alpha/CD172a Rat mAb (Biotin) - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1

Cat. No.: Ab191788

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

P84

Conjugation

Biotin

Overview

Rat anti Mouse SIRP alpha/CD172a Antibody, Monoclonal (P84), could be used for in vivo SIRPα blocking, in vitro SIRPα blocking, WB, IP, Flow and so on.

Specifications

Product Name

SIRP alpha/CD172a Rat mAb (Biotin) - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1

Synonyms

Bit | SIRP | SHP-1 | Ptpns1 | Idd13.2 | BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs | Brain Ig-like molecule with tyrosine-based activation motifs | CD172 antigen-like family member A | CD172a antigen | CD172a | Inhibitory

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial

Host species

Rat

Specificity

SIRP alpha/CD172a

Immunogen

Mouse brain membrane protein

Positive Control

Flow: C57BL/6 mouse bone marrow cells.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG1

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788)

SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were stained with SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788)

SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

SIRPA Tbio Tyrosine-protein phosphatase non-receptor type substrate 1 (0 Activities)

Discover Target: SIRPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)