Experiments on the effects of the ripening action of ethylene

Summary

Ethylene is one of the five major endogenous hormones and the only gaseous hormone. Almost all plant tissues have the potential to produce ethylene. Ethylene has a variety of physiological functions in plants, the most important of which include: promotion of fruit ripening, "triple response" to stem growth, acceleration of abscission and senescence, and induction of female flower formation.

Operation method

Experiments on the ripening effect of ethylene

Principle

Ethylene is one of the five major endogenous hormones and the only gaseous hormone. Almost all plant tissues have the potential to produce ethylene. Ethylene has a variety of physiological functions in plants, the most important of which include: promotion of fruit ripening, "triple response" to stem growth, acceleration of abscission and senescence, and induction of female flower formation. It has been known that ethylene can change membrane permeability and cell compartmentalization, and induce the synthesis, release or activation of many enzymes (especially degradative enzymes), which is one of the main reasons why ethylene promotes ripening and abscission. The synthesis of new RNAs and proteins is also often observed during the action of ethylene, suggesting that ethylene acts at the level of gene regulation. Exogenous ethylene has almost exactly the same physiological effects as endogenous ethylene, and is therefore used in production to regulate plant growth and development through exogenous ethylene. Ethylene is the most widely used synthetic ethylene releasing agent, its chemical name is 2 ── chloroethyl phosphate, after its entry into the plant body, under the conditions of pH>4, chemical decomposition occurs, releasing ethylene, the reaction formula is: In this experiment, the appropriate concentration of ethylene treatment can be used to promote the ripening of tomato or apple fruits, and to promote the formation of female flowers of cucumber, and the flowering of the early.

Materials and Instruments

Tomato Apple Fruit
Tween-20
Chromatography cylinder Beaker

Move

I. Materials and equipment

Tomato or apple fruit

Chromatography cylinder (or plastic bag), beaker

Drugs

0.1% Tween-20

Aqueous solution of vinblastine: take 1.25 and 0.05 M M 3 of "vinblastine" or "a test spirit" original liquid (containing 40% of the active ingredient) respectively, and then volume each to 1000 ml, i.e., it will be 500pp M and 200pp M aqueous solution.

Experimental steps

1. Select the maturity of the same, the skin began to change from green to white tomato fruit (or apple fruit with similar maturity) 30, are divided into three groups, respectively, immersed in 500pp M, 200pp M ethylene glycol aqueous solution and distilled water for 1 minute, immersion in the liquid add 0.1% Tween-20 as a wetting agent.

2. Take out the fruits and put them into three chromatographic cylinders (or plastic bags), seal them tightly, and place them in a dark place at 25℃-30℃.

Day by day to observe the color of the fruit, hardness and maturity of the process of change, record the maturity of the fruit and the number of ripe fruit, until all the fruit is ripe.

Results and Analysis

Try to show the relationship between the ripening effect of ethylene glycol and its concentration in the form of a graph or a table, and analyze the ripening effect of ethylene glycol based on the results of the experiment, explaining its possible mechanism.

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