Goat Anti-Human IgG (TRITC) - Secondary antibody

Cat. No.: Ab175836

GRADE & PURITY Validated, ExactAb™, Azide Free, Ex:557nm, Em:576nm, 1.0 mg/mL

Application

IF/ICC

Host species

Goat

Reactive species

Human

Conjugation

TRITC

Overview

Application
IF: 1:1000

Specifications

Product Name

Goat Anti-Human IgG (TRITC) - Secondary antibody

Synonyms

Goat Anti-Human Antibody (TRITC) | TRITC-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / TRITC antibody | Goat Anti-Human IgG-TRITC antibody

Specifications & Purity

ExactAb™, Validated, Azide Free, Ex:557nm, Em:576nm, 1.0 mg/mL

Host species

Goat

Conjugation

TRITC

Excitation (nm)

Green Laser(532nm)

Emission(nm)

576nm

Ex/Em(nm)

Ex:557nm;Em:576nm

Grade

Azide Free, Em:576nm, Ex:557nm, ExactAb™, Validated

Purification Method

Antigen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

IF: Goat Anti-Human IgG (TRITC) (Ab175836)

Goat Anti-Human IgG (TRITC) (Ab175836) - IF
IF analysis of EGFR (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Necitumumab (anti-EGFR) (Ab169264) at 1 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (TRITC) (Ab175836) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• IF/ICC:

  1:1000;