HA tag Mouse mAb - Primary antibody, specific to HA, IgG2b

Cat. No.: Ab106781

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

ELISA, IF/ICC, IP, WB

Host species

Mouse

Reactive species

Species independent

Isotype

Mouse IgG2b

Clone Number

12CA5

Conjugation

Unconjugated

Overview

Mouse anti HA tag Antibody, Monoclonal antibody(12CA5), could be used for WB, ICC, IF, IP, ELISA and so on.
Application
WB: 1/1000 - 1/5000
ICC/IF: 1/1000 - 1/5000
IP: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Specifications

Product Name

HA tag Mouse mAb - Primary antibody, specific to HA, IgG2b

Synonyms

HA epitope tag antibody | HA1 antibody | HA2 antibody | hemagglutinin antibody | Hemagglutinin HA1 chain antibody | Hemagglutinin HA2 chain antibody | HA Epitope Tag | HA Tag | HA | Hemagglutinin | HA tag antibody | Anti- HA tag antibody

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Mouse

Specificity

HA

Immunogen

Influenza virus hemagglutinin protein (AA 98-106).

Positive Control

WB: HEK293 cells transfected with HA tag.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

IgG2b

SDS-PAGE

26.1 kDa (Light Chain) & 52.3 kDa (Heavy Chain), under reducing conditions; 209.0 kDa, under non-reducing conditions.

Purification Method

Protein G purified

Concentration

See COA

Note

This product is an alternative to Ab106776

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Western Blot: HA tag Mouse mAb (Ab106781)

HA tag Mouse mAb (Ab106781) - Western Blot
All lanes: HA tag Mouse mAb (Ab106781) at 1.0 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 115 kDa
Observed band size: 66, 120 kDa
Exposure time: 0.4 s

Application

• Application: Dilution info
• WB:

  1/1000 - 1/5000 ;

• IP:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

• ICC/IF:

  1/1000 - 1/5000