HMGB2 Antibody - Primary antibody, specific to HMGB2, Rabbit IgG

Cat. No.: Ab108314

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Cow, Human, Mouse

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human HMGB2 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

HMGB2 Antibody - Primary antibody, specific to HMGB2, Rabbit IgG

Synonyms

C80539 antibody | High mobility group (nonhistone chromosomal) protein 2 antibody | High mobility group box 2 antibody | High mobility group protein 2 antibody | High mobility group protein B2 antibody | HMG 2 antibody | HMG B2 antibody | HMG-2 antibody |

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemical and Physiological Mechanisms

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding

Host species

Rabbit

Specificity

HMGB2

Immunogen

A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HMGB2.

Positive Control

WB: HEL and Jurkat cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: HMGB2 Antibody (Ab108314)

HMGB2 Antibody (Ab108314) - Western Blot
All lanes: HMGB2 Antibody (Ab108314) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/30000 dilution

Predicted band size: 24 kDa
Observed band size: 27 kDa
Exposure time: 60.0 s

IHC: HMGB2 Antibody (Ab108314)

HMGB2 Antibody (Ab108314) - IHC
Immunohistochemical analysis of HMGB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with HMGB2 Antibody (Ab108314) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: HMGB2 Antibody (Ab108314)

HMGB2 Antibody (Ab108314) - IF/ICC
Immunofluorescent analysis of HMGB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with HMGB2 Antibody (Ab108314) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IF/ICC:

  1/100-1/500 ;

• IHC:

  1/100-1/200;

Associated Targets(Human)

HMGB2 Tbio High mobility group protein B2 (0 Activities)

Discover Target: HMGB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action